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http://hdl.handle.net/11320/16572
Tytuł: | Recombinant Human Plasma Gelsolin Stimulates Phagocytosis while Diminishing Excessive Inflammatory Responses in Mice with Pseudomonas aeruginosa Sepsis |
Autorzy: | Piktel, Ewelina Wnorowska, Urszula Cieśluk, Mateusz Deptuła, Piotr Prasad, Suhanya V. Król, Grzegorz Durnaś, Bonita Namiot, Andrzej Markiewicz, Karolina H. Niemirowicz-Laskowska, Katarzyna Wilczewska, Agnieszka Z. Janmey, Paul A. Reszeć, Joanna Bucki, Robert |
Słowa kluczowe: | plasma gelsolin inflammation phagocytosis sepsis Pseudomonas aeruginosa |
Data wydania: | 2020 |
Data dodania: | 28-maj-2024 |
Wydawca: | MDPI |
Źródło: | International Journal of Molecular Sciences, Volume 21, Issue 7 (2020), p. 1-19 |
Abstrakt: | Plasma gelsolin (pGSN) is a highly conserved abundant circulating protein, characterized by diverse immunomodulatory activities including macrophage activation and the ability to neutralize pro-inflammatory molecules produced by the host and pathogen. Using a murine model of Gram-negative sepsis initiated by the peritoneal instillation of Pseudomonas aeruginosa Xen 5, we observed a decrease in the tissue uptake of IRDye®800CW 2-deoxyglucose, an indicator of inflammation, and a decrease in bacterial growth from ascitic fluid in mice treated with intravenous recombinant human plasma gelsolin (pGSN) compared to the control vehicle. Pretreatment of the murine macrophage line RAW264.7 with pGSN, followed by addition of Pseudomonas aeruginosa Xen 5, resulted in a dose-dependent increase in the proportion of macrophages with internalized bacteria. This increased uptake was less pronounced when cells were pretreated with pGSN and then centrifuged to remove unbound pGSN before addition of bacteria to macrophages. These observations suggest that recombinant plasma gelsolin can modulate the inflammatory response while at the same time augmenting host antibacterial activity. |
Afiliacja: | Ewelina Piktel - Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok Urszula Wnorowska - Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok Mateusz Cieśluk - Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok Piotr Deptuła - Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok Suhanya V. Prasad - Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok Grzegorz Król - Department of Microbiology and Immunology, the Faculty of Medicine and Health Sciences of the Jan Kochanowski University in Kielce Bonita Durnaś - Department of Microbiology and Immunology, the Faculty of Medicine and Health Sciences of the Jan Kochanowski University in Kielce Andrzej Namiot - Department of Anatomy, Medical University of Bialystok Karolina H. Markiewicz - Institute of Chemistry, University of Białystok Katarzyna Niemirowicz-Laskowska - Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok Agnieszka Z. Wilczewska - Institute of Chemistry, University of Białystok Paul A. Janmey - Institute for Medicine and Engineering, University of Pennsylvania Joanna Reszeć - Department of Pathology, Medical University of Bialystok Robert Bucki - Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok; Department of Microbiology and Immunology, the Faculty of Medicine and Health Sciences of the Jan Kochanowski University in Kielce |
E-mail: | Ewelina Piktel: ewelina.piktel@wp.pl Urszula Wnorowska: u.wnorowska@gmail.com Mateusz Cieśluk: mticv1@gmail.com Piotr Deptuła: piotr.deptula@umb.edu.pl Suhanya V. Prasad: suhanyavp@gmail.com Grzegorz Król: g.krol@op.pl Bonita Durnaś: bonita.durnas@onkol.kielce.pl Andrzej Namiot: anamiot@poczta.onet.pl Karolina H. Markiewicz: k.markiewicz@uwb.edu.pl Katarzyna Niemirowicz-Laskowska: katia146@wp.pl Agnieszka Z. Wilczewska: agawilczuwb@gmail.com Paul A. Janmey: janmey@pennmedicine.upenn.edu Joanna Reszeć: joannareszec@gmail.com Robert Bucki: buckirobert@gmail.com |
Sponsorzy: | This work was supported by the National Science Center, Poland under Grant: UMO-2015/17/B/NZ6/03473 (to RB), National Institutes of Health: GM111942 (to PAJ) and Medical University of Bialystok: SUB/1/DN/19/001/1162 (to RB), N/ST/MN/18/001/1162 (to MC). Part of the study was conducted with the use of equipment purchased by the Medical University of Białystok as part of the RPOWP 2007-2013 funding, Priority I, Axis 1.1, contract No. UDA- RPPD.01.01.00-20-001/15-00 dated 26.06.2015. This work was supported by the program of the Minister of Science and Higher Education under the name “Regional Initiative of Excellence in 2019–2022”, project number: 024/RID/2018/19, financing amount: 11.999.000,00 PLN. |
URI: | http://hdl.handle.net/11320/16572 |
DOI: | 10.3390/ijms21072551 |
ISSN: | 1422-0067 |
metadata.dc.identifier.orcid: | brakorcid brakorcid 0000-0002-5931-4376 0000-0003-3553-3774 brakorcid brakorcid brakorcid brakorcid brakorcid 0000-0002-3311-7147 brakorcid brakorcid brakorcid 0000-0001-7664-9226 |
Typ Dokumentu: | Article |
metadata.dc.rights.uri: | http://creativecommons.org/licenses/by/4.0/ |
Właściciel praw: | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
Występuje w kolekcji(ach): | Artykuły naukowe (WChem) |
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E_Piktel_U_Wnorowska_M_Ciesluk_KH_Markiewicz_at_al_Recombinant_Human_Plasma_Gelsolin_Stimulates.pdf | 3,23 MB | Adobe PDF | Otwórz |
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