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    • Proszę używać tego identyfikatora do cytowań lub wstaw link do tej pozycji: http://hdl.handle.net/11320/16603
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    Pole DCWartośćJęzyk
    dc.contributor.authorMisiak, Paweł-
    dc.contributor.authorNiemirowicz-Laskowska, Katarzyna-
    dc.contributor.authorMarkiewicz, Karolina H.-
    dc.contributor.authorMisztalewska-Turkowicz, Iwona-
    dc.contributor.authorWielgat, Przemysław-
    dc.contributor.authorKurowska, Izabela-
    dc.contributor.authorSiemaszko, Gabriela-
    dc.contributor.authorDestarac, Mathias-
    dc.contributor.authorCar, Halina-
    dc.contributor.authorWilczewska, Agnieszka Z.-
    dc.date.accessioned2024-06-04T09:31:54Z-
    dc.date.available2024-06-04T09:31:54Z-
    dc.date.issued2020-
    dc.identifier.citationInternational Journal of Nanomedicine, Vol. 15, 2020, p. 7263-7278pl
    dc.identifier.issn1176-9114-
    dc.identifier.urihttp://hdl.handle.net/11320/16603-
    dc.description.abstractPurpose: Efficient intracellular delivery of a therapeutic compound is an important feature of smart drug delivery systems (SDDS). Modification of a carrier structure with a cellpenetrating ligand, ie, cholesterol moiety, is a strategy to improve cellular uptake. Cholesterol end-capped poly(N-isopropylacrylamide)s offer a promising foundation for the design of efficient thermoresponsive drug delivery systems. Methods: A series of cholesterol end-capped poly(N-isopropylacrylamide)s (PNIPAAm) with number-average molar masses ranging from 3200 to 11000 g·mol–1 were synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization from original xanthate-functionalized cholesterol and self-assembled into micelles. The physicochemical characteristics and cytotoxicity of cholesterol end-capped poly(N-isopropylacrylamide)s have been thoroughly investigated. Results: Phase transition temperature dependence on the molecular weight and hydrophilic/hydrophobic ratio in the polymers were observed in water. Biological test results showed that the obtained materials, both in disordered and micellar form, are non-hemolytic, highly compatible with fibroblasts, and toxic to glioblastoma cells. It was found that the polymer termini dictates the mode of action of the system. Conclusion: The cholesteryl moiety acts as a cell-penetrating agent, which enables disruption of the plasma membrane and in effect leads to the restriction of the tumor growth. Cholesterol end-capped PNIPAAm showing in vitro anticancer efficacy can be developed not only as drug carriers but also as components of combined/synergistic therapy.pl
    dc.language.isoenpl
    dc.publisherDove Medical Presspl
    dc.rightsUznanie autorstwa-Użycie niekomercyjne 4.0 Międzynarodowe*
    dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
    dc.subjectcholesterol-end capped poly(N-isopropylacrylamide)pl
    dc.subjectcell-penetrating moleculespl
    dc.subjectthermoresponsive polymer micellespl
    dc.subjectdrug carrierspl
    dc.titleEvaluation of Cytotoxic Effect of Cholesterol End-Capped Poly(N-Isopropylacrylamide)s on Selected Normal and Neoplastic Cellspl
    dc.typeArticlepl
    dc.rights.holder© 2020 Misiak et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms. php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).pl
    dc.identifier.doi10.2147/ijn.s262582-
    dc.description.EmailAgnieszka Z. Wilczewska: agawilcz@uwb.edu.pl-
    dc.description.EmailKarolina H. Markiewicz: k.markiewicz@uwb.edu.pl-
    dc.description.AffiliationPawel Misiak - Faculty of Chemistry, University of Bialystok, Bialystok, Polandpl
    dc.description.AffiliationKatarzyna Niemirowicz-Laskowska - Department of Experimental Pharmacology, Medical University of Bialystok, Bialystok, Polandpl
    dc.description.AffiliationKarolina H. Markiewicz - Faculty of Chemistry, University of Bialystok, Bialystok, Polandpl
    dc.description.AffiliationIwona Misztalewska-Turkowicz - Faculty of Chemistry, University of Bialystok, Bialystok, Polandpl
    dc.description.AffiliationPrzemysław Wielgat - Department of Clinical Pharmacology, Medical University of Bialystok, Bialystok, Poland;pl
    dc.description.AffiliationIzabela Kurowska - Faculty of Chemistry, University of Bialystok, Bialystok, Poland; Doctoral School of Exact and Natural Sciences, University of Bialystok, Bialystok, Poland;pl
    dc.description.AffiliationGabriela Siemiaszko - Faculty of Chemistry, University of Bialystok, Bialystok, Polandpl
    dc.description.AffiliationMathias Destarac - 5IMRCP, CNRS UMR 5623, Université de Toulouse, Toulouse, Francepl
    dc.description.AffiliationHalina Car - Department of Experimental Pharmacology, Medical University of Bialystok, Bialystok, Polandpl
    dc.description.AffiliationAgnieszka Z. Wilczewska - Faculty of Chemistry, University of Bialystok, Bialystok, Polandpl
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    dc.description.volume15pl
    dc.description.firstpage7263pl
    dc.description.lastpage7278pl
    dc.identifier.citation2International Journal of Nanomedicinepl
    dc.identifier.orcid0000-0002-6882-3519-
    dc.identifier.orcid0000-0002-3311-7147-
    dc.identifier.orcid0000-0002-4857-0489-
    dc.identifier.orcid0000-0001-6191-6053-
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